Analysis of Arg aminoacyl synthetase.

Analysis performed on 2024-09-30 10:19:54

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 46029 559 45470 26531
Archaea 3161 92 3069 1386
Vertebrata 1641 405 1236 473
Mammals 604 54 550 131

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
660 21 583

Domains

Domains
Current Study 1, 73
Guo et al (2010) 5, 32 | 41, 64

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 588 72 660 8.166667
Number of Variants 528 76 604 6.947368
Number of Pathogenic Variants 16 5 21 3.200000
Number of Benign/Unknown Variants 512 71 583 7.211268
Fisher exact test 0.167580

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 608 52 660 11.692308
Number of Variants 553 51 604 10.843137
Number of Pathogenic Variants 19 2 21 9.500000
Number of Benign/Unknown Variants 534 49 583 10.897959
Fisher exact test 0.695035

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 588 72 0.89 (0.00) 0.78 (0.02) 9.293e-01 7.893e-01 28792.00 2.98e-07
Guo et al (2010) 608 52 0.88 (0.00) 0.82 (0.02) 9.271e-01 8.940e-01 18941.00 8.80e-03

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains